Purified Anti-Human CD166 Antibody[3A6], Functional Grade (AN004260)
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For research use only.
| Alternate Names | Activated leukocyte cell adhesion molecule, CD166, CD166 antigen, MEMD, ALCAM |
| Clone | |
| Verified Samples | Verified Samples in FCM: Human peripheral blood monocytes cells |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Block Reported in the literaturee |
| Background | CD166, also known as the CD6 ligand or the Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a 100-105 kD transmembrane glycoprotein. It belongs to the Ig superfamily of proteins and expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently CD166 has also been used as a potential cancer stem cell marker. The antibody reacts with human activated leukocyte cell adhesion molecule (ALCAM). None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Human CD166 protein |
| Abbre | CD166 |
| Swissprot | |
| Host | Mouse |
| Reactivity | Human |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG1, κ |
| Isotype Control | Purified Mouse IgG1, κ Isotype Control[MOPC-21], Functional Grade |
| Applications |
FCM;Block
|
| Research Areas | Tags and Cell Markers;Immunology;CDs |
| Cellular Localization |
Cell membrane, Cell projection, Membrane, Secreted
|
| Tissue Specificity |
Detected on hematopoietic stem cells derived from umbilical cord blood.
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Ferragut F, et al. Cytokine Growth Factor Rev. 2021;61:27-37. |
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